Globin Gene Switching

Globin gene switching in transgenic mice carrying HS2-globin gene constructs.

We have examined the pattern of human globin gene switching in transgenic mice containing three different gamma and beta gene constructs (HS2G gamma A gamma delta beta, HS2A gamma beta neo, and HS2A gamma en beta) and compared the results with previously described transgenics (HS2A gamma beta, HS2G gamma A gamma-117 delta beta, and LCR epsilon G gamma A gamma delta beta). Developmental regulati...

DNA methylation: a secondary event in globin gene switching?

Changes in gamma-globin gene methylation accompany the fetal to adult globin switch in man. Using somatic cell hybrids made by fusing mouse erythroleukemia and human fetal erythroid cells, we asked whether methylation is a cause or a consequence of gamma-gene inactivation during development. These hybrids initially express human gamma-globin but switch with time in culture to adult globin gene ...

Chicken globin gene number.

Using complementary DNA prepared from adult chicken globin messenger RNA, we show the existence of 2-3 non-cross-hybridising globin sequences in the chicken genome, each of which is present in only one copy per haploid genome. This was done by solution hybridization to total DNA under conditions of cDNA excess. The data agreses with results of RNA-driven hybridisation of globin complementary DN...

-globin gene families

Gabriela Aguileta *, Joseph P. Bielawski 3,4 and Ziheng Yang 1 1 Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, England 2 Linnaeus Centre for Bioinformatics, University of Uppsala, BMC, Box 598, 75124, Uppsala, Sweden 3 Department of Biology, Dalhousie University, Halifax, Nova Scotia, B3H 4J1, Canada 4 Department of Mathematics & Statistics, D...

Effects of Butyrate and Glucocorticoids on g- to b-Globin Gene Switching in Somatic Cell Hybrids